Expected Outcome: Proposals are expected to contribute to all the following expected outcomes: the mechanistic understanding of PFAS (per- and polyfluoroalkyl substances) effects on biodiversity, on ecosystems and on ecosystem services is improved and evaluations of their extent are available; research needs to close knowledge gaps and to address issues on biodiversity and ecosystem services are better identified; the new knowledge can be integrated by policymakers in their decisions related to biodiversity protection and restoration, environmental protection and protection of human health as well as in socio-economic cost-benefit analysis for policy measures on PFAS. Scope: PFAS pollution significantly impacts human health and the environment. Addressing PFAS is notably a flagship action under the Water Resilience Strategy [1] . There is growing evidence on impacts on biodiversity above and below ground and water, but more evidence is needed to substantiate the effects on different species, populations and entire ecosystems, and their extent. A European Commission study published on 29 January 2026 [2] revealed significant knowledge gaps on the effects of PFAS on biodiversity. In
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